In Vitro Fertilisation (IVF) and Culture (IVC)

Maximise Your Outcomes

In Vitro Fertilisation (IVF) is a cornerstone of Evogene’s advanced reproductive technology. The process begins with the OPU and collection of oocytes from the donor. These oocytes are then matured in a controlled laboratory environment and fertilised 20–24 hours later with carefully selected semen under controlled laboratory conditions.

Depending on your breeding goals, we offer flexible fertilisation options—including fresh, frozen-thawed, conventional and sexed semen. The team process the semen to maximise outcomes and minimise wastage. The developing embryos are cultured in optimal conditions for seven days, after which the highest quality embryos are transferred into recipients

Why choose IVF?

No late-night donor AI’s required in the field
Use less semen per fertilization by:

Inseminating multiple donors with a single straw
Using a partial straw over multiple donors or IVF days

Split the donors’ oocytes across multiple sires
Scientists select only the most motile and viable sperm
Fertilisation occurs in vitro, under strict laboratory control
Successful embryo production from contaminated semen that has a history of failing in conventional AI and flushing

Why is IVF important?

IVF is a powerful tool for accelerating genetic gain, improving reproductive efficiency, and making the most of every donor and every straw of semen. By producing multiple embryos from a single collection and enabling precise control over genetics, IVF transforms high-value breeding plans into high-impact outcomes - efficiently, economically, and reliably.

How are oocytes fertilised in the lab?

In the laboratory, oocytes collected via OPU are carefully fertilised under highly controlled conditions to give each egg the best chance of developing into a healthy embryo. A critical part of this process is semen preparation.
Before fertilisation:

Semen is thoroughly processed to remove cryoprotectants, seminal plasma, and any contaminants that could affect fertilisation or embryo development.
Only the highest-quality sperm—with optimal motility, morphology, and vitality—are selected for use.
Sperm are added to the oocytes at the precise concentration needed to maximise fertilisation while minimising polyspermy.

This meticulous preparation ensures that every oocyte is exposed to sperm in ideal conditions, stacking the odds in favour of successful fertilisation and high-quality embryo development.

How long does the IVF and IVC process take?

The IVF and IVC process is precise and takes several days to ensure the best outcomes for embryo development. A typical timeline looks like this:

Day -1: OPU (Ovum Pick-Up) – Eggs are collected from the donor via ultrasound-guided aspiration.
Day 0: IVF (In Vitro Fertilisation) – The collected oocytes are fertilised in the laboratory with carefully prepared, high-quality sperm.
Days 1–7/8: IVC (In Vitro Culture) – Fertilised oocytes are cultured under controlled conditions, allowing embryos to develop to the blastocyst stage.

While it may feel like a long process, each step is essential to give every oocyte the best chance to develop into a healthy, viable embryo. The attention to detail during collection, fertilisation, and culture is what maximises your program’s success.

How will I find out the progress?

At Evogene, we understand how important it is to stay informed throughout the IVF and embryo culture process. Our dedicated team provides regular updates on key milestones, including cleavage rates and embryo production.
If any concerns arise at any stage, we will contact you immediately, ensuring you are fully informed and confident in every step of your program. Additionally, all clients have access to our special Evogene platform, where you can securely view:

Your entire inventory
Program results
Embryo production certificates

This platform gives you real-time access to all the details of your program, providing transparency, reassurance, and convenience from start to finish.